is specifically designed for the measurement of intracellular calcium or ph changes in live cells through the simultaneous detection of two different fluorescence emission spectra intensities. fluorescent dyes are excited by one excitation spectrum producing simultaneously two emission spectra. the resulting a and b signals indicate changes within the cell. a ratio signal is provided for a:b or b:a.
static or kinetic analysis
hplc high pressure liquid chromatography
low pressure liquid chromatography
range：200-700nm (standard)，200-900nm (available)
sensitivity：full scale, 10 picogram quinine, sulfate (10.0ppt) with instrument noise, less than 5% in respect to full scale.
linearity： +1% of full scale
two digital displays: 000-1999
two recorder signal outputs: 0 to 2 volts
power: 220 vac., 0.8 amp at 50/60 hz.
dimensions wdh: 39.4×44.5×21.6cm, 19.5 kg;
optical system: exclusive seven element quartz optical system focuses excitation energy onto the sample with emmitted fluorescence energy collected and focused to two (2) photomultiplier tubes providing two (2) individual emission channels. excitation and emission filters are placed in collimated (parallel) light paths for optimum filter performance.
optical filters: excitation and emission filter holders are readily accessible to accommodate glass and/or interference filters. filters covering the entire fluorescent spectral range are available.
light source: 100 watt high intensity mercury vapor lamp radiates the full uv and visible spectrum of energy.
photomultiplier tubes: two 200-700nm photomultiplier tubes are standard. extended range (200-900nm) photomultiplier tube is also available.
blank suppression and variable gain: two sets of front panel controls are provided for the suppression of blank fluorescence background to zero and adjustment of full scale signal to a specific number.
display: two independent fluorescent intensity levels are digitally displayed and may be recorded simultaneously with a recorder signal.
ratio output: a ratio signal is provided for a:b or b:a.
note: when used in l rather than t configuration, operation and performance is identical to the model a1 fluorometer.
dual emission fluorometer ordering information
model t-em filter fluorometer
• 100 watt high intensity mercury vapor lamp
• seven element all quartz optical system
• two sets of electronic blank suppression controls
• two sets of sensitivity ranges - .001, .01, .1, 1.0
• two digital displays 000-1999
• two se-lected photomultiplier tubes 200-700 nm
six position excitation aperture wheel
• one excitation and two emission filter holders
• three position carrier for 75 x 10mm round cells
• two output signals for a and b channel＊
• ratio output signal a:b or b:a＊
• 115 vac., 50/60 hz operation
note: 220 vac. operation available upon request
accessories and attachments
constant temperature assembly
strip chart recorder
se-lected photomultiplier tubes
alternate illumination source
hplc flow cell
large volume flow cells
three polarizer filter holder attachments